Single-cell manipulation devices

Isolation of single living cells for manipulation and analysis creates the ultimate control condition, opening a window to cellular biology unadulterated by the myriad of structures and factors present in the organism and its environment. A multidisciplinary consortium created single-cell manipulation (SCM) technology in a prior FP7 project, PASCA. With EU support of the SICTEC (Single cell technologies for SMEs) project, the team is supporting the development of innovative products and applications based on the proven SCM prototype instrument by participating small and medium-sized enterprises (SMEs) and businesses.

The SCM technology utilises inkjet-type printing of single live biological cells in microdroplets. A technician places the sample in a well via a micropipette after which the instrument, under full visual control, ejects individual droplets onto or into the substrate only if a single cell is present. If no cells or more than one cell is present in the droplet, the droplet is automatically discarded. A robotic platform capable of 3D motion and three cameras for full vison control manipulates the sample and facilitates visualisation throughout the entire process.

SICTEC is validating and extending its use with careful attention to factors affecting commercialisation. Designs for all intended applications have been manufactured and validated within the first reporting period. In addition, major work enabled quadrupling the yield of high-quality disposable elements used for dispensing the samples, and an additional 10-fold increase is expected in the next reporting period. Two new print heads target applications in single-bacterium dispensing and fluorescence detection of labelled cells and novel liquid handling devices are currently being optimised.

The project's five SME and two research and technology development partners are poised to exploit the revolutionary SCM technology in pioneering systems, devices and applications. The project website presents videos of the prototype at work and invites others to become pilot partners in experiments and applications. Benefits will abound for basic and applied research as well as for biomedicine and the well-being of EU citizens.